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KMID : 0613820040140050834
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Journal of Life Science
2004 Volume.14 No. 5 p.834 ~ p.839
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The Improvement of surface activity and Emulsification Activity by Transformation of Lipase Gene in Klebsiella sp. KCL-1, Oil-Degrading Bacterium
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Jung Soo-Yeol
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Abstract
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To improve and oil degrading activity, the lipase gene from Pseudomonase sp. was transformed into Klebsiella sp. KCL-1, an oil degrading bacterium. The selected trasformant was named as a KCL-1/pET-Lip. The surface tension of culture broth of KCL-1/pET-Lip was decreased to 33 dyne/cm from 55 dyne/cm using 4% (v/v) soybean oil as sole carbon source. The surface tension were 44 and 37.5 dyne/cm, to 2% (w/v) glucose and 4% (v/v) kerosene medium, respectively. The emulsification activity of the biosurfactant solution containing lipase of KCL-1/ pET-Lip improved better than wild type KCL-1. The soybean oil was most efficient carbon source and substrate for surface activity and emulsification activity of KCL-1/pET-Lip. The expression of lipase was confirmed by SDS-PAGE.
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KEYWORD
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Klebsiella sp, KCL-1, pET-Lip, surface tension, surface activity, emulsification activity
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